Services

The following services are available for the Lentiviral Laboratory.
To schedule for services, please contact Dr. Liana Asatryan at asatryan@pharmacy.usc.edu.

Services provided include

  • Backbone vectors for cDNA expression
  • Backbone vectors for shRNA expression
  • Development of lentiviral constructs
  • Small-scale lentivirus production
  • Transductions of primary cells and tissue culture
  • Production of stable cell lines
  • Consultation for vector modalities, troubleshooting, support documentation for grants

Services turnaround time

  • Lentiviral vector construction – 6-8 weeks
  • Lentivirus packaging and concentration (small scale) – 10 days
  • Lentiviral stock titrations – 2 weeks
  • Lentiviral infections of primary cells including neurons – 2-4 days

Backbone vectors for cDNA expression

  • pLVX-AcGFP1-C1
  • pLVX-AcGFP1-N1
  • pLVX-IRES-ZsGreen1
  • pLVX-IRES-ZsGreen1-C1
  • pLVX-IRES-ZsGreen1-N1

Backbone vectors for shRNA expression

  • pLVX-shRNA1
  • pLVX-shRNA2